ECOLOGIENA for Environmental Pollutants
Nonionic Surfactants APE ELISA Kit


  • The monoclonal antibody binds exclusively with Alkylphenol Ethoxylate (APE) and Alkylphenol ethoxy acetic acid, and does not cross-react with other surfactants or compounds of similar structure.
  • The detection range is between 0.02mg/L and 1.0mg/L. A simple concentration protocol based on solid phase extraction is available to determine much lower concentration.
  • With ease of handling, the total time for measurement is only 2.5 hours.
  • The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost.


APE is an abbreviation for Alkylphenol Ethoxylate, also called polyoxyethylene alkylphenyl ether.
APE belongs to nonionic surfactant and is used mainly for industrial detergent or emulsifier. Ethylene oxide chains in APE are subject to microbial decomposition, generating toxic and hydrophobic biodegraded compounds.
APE has recently been pointed out as a precursor of endocrine disrupting chemicals.
Octylphenol ethoxylate(N=8) and nonylphenol ethoxylate(n=9) are the representative APEs.
Chemical Structure

APE Standard Curve

APE Standard Curve Samples containing APE within the dynamic range (0.02mg/L- 1.0mg/L) can be directly applied to assay after filtration.
Samples with APE content below the range must be concentrated with solid phase extraction prior to the ensuing session.
Coefficient of variation(CV) is generally under 10% throughout the dynamic range..

Pretreatment (Simplified Solid Phase Extraction)

Pretreatment (Simplified Solid Phase Extraction) With this concentration protocol, APE is measurable around the order of parts per trillion, or ng/mL.
Pour sample liquid through a C18 solid-phase cartridge and elute APE with methanol for concentration.
This protocol assures the simultaneous extraction of AE, another POE type nonionic surfactant, and removal of substances that may obstruct APE binding.
Reconstitute the elute in 10% methanol aqueous solution after evaporation for the ELISA assay.
  • The specific procedure may vary in sample quality.

Cross-Reactivity Pattern

Compound % reactivity
Nonionic surfactants
NPE(Nonylphenol ethoxylates)
NP20EO (Ave. EO chain length 20) 79
NP15EO (Ave. EO chain length 15) 73
NP10EO (Ave. EO chain length 10) 100
NP5EO (Ave. EO chain length 5) 82
NP2EO 32
NP1EO 14
OPE(Octhylphenol ethoxylates) @
OP20EO (Ave. EO chain length 20) 92
OP15EO (Ave. EO chain length 15) 129
OP10EO (Ave. EO chain length 10) 200
OP5EO (Ave. EO chain length 5) 244
OP2EO 129
Nonylphenol ethoxy acetic acid (NPnEC)
NP2EC 267
NP1EC 200
AE (Alkyl ethoxylates) <0.2
Anionic surfactants
LAS (Linear alkylbenzene sulfonate) <0.2
SOAP (Sodium laurate) <0.2
SDS (Sodium dodecyl sulfate) <0.2
AES (Alkylether sulfate) <0.2
Substances of similar structure
Phenol <0.2
PEG (Polyethylene glycol) <0.2
The monoclonal antibody has a high specificity to APE with various polyethoxylic chain length (n=1-22) and doesn't cross-react with other surfactants or compounds of similar structure.

Kit Format

Kit Format Comment
Microplate 1 plate (96 wells)
& reagents
Needs a microplate reader(450nm)
For multiple and simultaneous measurement

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