ECOLOGIENA for Environmental Pollutants
17β-Estradiol (E2) ELISA Kit

17β-Estradiol (E2) ELISA Kit

  • E2 monoclonal antibody binds exclusively with E2 and does not show cross-reaction with other chemicals of similar structures. A monoclonal antibody is uniform in quality, generating very little lot-to-lot variation.
  • The quantitative analysis ranges from 0.05μg/L to 1μg/L (ppb).
  • The ELISA measurement is highly reproducible; the coefficient of variation (CV) is mostly under 10%.
  • The assay requires less amount of harmful solvent than instrument analyses.
  • With ease of handling, the total time for measurement is only 2.5 hours.
  • The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost.

Chemical Structure Standard Curves
Chemical Structures of Targets Standard Curves
Pretreatment Methods
See here.

Comparison with LC/MS/MS

Averaged value of E2 (ng/L)
Sample Na) LC-MS/MS E2-Ecologienab) E2-ADc) E2-CCc) E2-NGc) E2-RBc)
Primary effluent 10 8.4 12.5 21.1 81.4 97.6 202.2
Aeration tank 13 1.9 2.1 4.4 9.4 7.8 31.6
Secondary effluent 5 1.2 2.6 5.3 15.1 18.8 75.9
a)
N=number of samples
b)
Ecologiena E2 kit
c)
Other kits on the market (for biological samples)

Cross-reactivity Pattern

Estrogens
Compound % Reactivity
E2 ELISA Kit
Estron (E1) 1.3
2-methoxy E1 <0.4
E1-3-sulfate 1.0
17β-Estradiol (E2) 100.0
16-keto E2 16.0
2-methoxy E2 2.0
E2-17-glucronide <0.4
E2-3-glucronide 16.0
E2-3-sulfate-17-glucronide <0.4
Estriol (E3) 0.6
16-epi-E3 0.5
E3-16-glucronide <0.4
　
Other hormones
Compound % Reactivity
E2 ELISA Kit
cis -Androsterone <0.03
trans -Androsterone <0.03
Cholesterol 0.46
Dehydroisoandrosterone <0.03
5α-Dihydroteststerone 0.38
Hydrocortisone 0.38
Pregnenolone 0.35
Teststerone <0.03

Kit Format

Kit Format Comment
Microplate 1 plate (96 wells)& reagents Needs a microplate reader (450nm)
For multiple and simultaneous measurement

For research use only. Not for diagnostic use.



The details of ECOLOGIENA are here.