ECOLOGIENA for Environmental Pollutants Total Estrogen (E1/E2/E3) ELISA Kit

Total Estrogen (E1/E2/E3) ELISA Kit

• ES monoclonal antibody binds exclusively with E1, E2 and E3, and does not show cross-reaction with other chemicals of similar structures. A monoclonal antibody is uniform in quality, generating very little lot-to-lot variation.
  
• The quantitative analysis ranges from 0.1μg/L to 3μg/L (ppb).
  
• The ELISA measurement is highly reproducible; the coefficient of variation (CV) is mostly under 10%.
  
• The assay requires less amount of harmful solvent than instrument analyses.
  
• With ease of handling, the total time for measurement is only 2.5 hours.
  
• The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost.

Chemical Structures of Targets

Standard Curves

Pretreatment Methods
See here.

Test Data

Recovery Test using River Water (n=4)

ES ELISA Kit Unspiked Determination (ng/L) 12.4 SD (%) 6.5 Spiked with 2ng/L E2 Determination (ng/L) 14.4 SD (%) 9.2 Recovery (%) (n=4) 100.0

Comparison with LC/MS/MS

Cross-reactivity Pattern

Other hormones

Compound % Reactivity ES ELISA Kit cis -Androsterone <0.03 trans -Androsterone <0.03 Cholesterol <0.03 Dehydroisoandrosterone <0.03 5α-Dihydroteststerone <0.03 Hydrocortisone <0.03 Pregnenolone <0.03 Teststerone <0.03

Kit Format

Kit Format		Comment
Microplate	1 plate (96 wells)& reagents	Needs a microplate reader (450nm) For multiple and simultaneous measurement