ECOLOGIENA for Environmental Pollutants
Anionic Surfactant LAS ELISA Kit |
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LAS ELISA Kit
- The monoclonal antibody binds exclusively with LAS and does not
cross-react with other surfactants or compounds of similar structure.
- The detection range is between 0.02mg/L and@1mg/L, sensitive
enough to render harmful solvent extraction and concentration unnecessary.
- A simple filtration through glass filter is the only pretreatment
before measurement.
- With ease of handling, the total time for measurement is only
2.5 hours.
- The kit, a 96-well microplate format, enables simultaneous measurement
of multiple samples at more reasonable cost.
LAS
| LAS is an abbreviation for
Linear Alkylbenzene
Sulfonate, the most widely used synthetic
detergent and the major component among methylene blue active substances (MBAS).
LAS has been pointed out to generate fish toxicity through bioaccumulation. |
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LAS Standard Curve
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The sample containing LAS within the
dynamic range (0.02mg/L-1mg/L) can be directly applied to assay after filtration.
Coefficient of variation (CV) is generally under 10% throughout the dynamic range.
- A concentration is necessary for a sample with LAS content below
the range for quantification.
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Comparison with Conventional Methods
ELISA exhibits high correlation with MBAS and HPLC. The value
from ELISA is almost equivalent to that of HPLC, which measures only LAS, and
is smaller than that of MBAS detecting other anionic surfactants as well.
Cross-Reactivity Pattern
| Compound |
% reactivity |
| Anionic surfactants |
 Linear
alkylbenzene sulfonates(LAS) |
| C12 |
100 |
| C11 |
87 |
| C10 |
101 |
| C9 |
114 |
| C8 |
75 |
| Sodium
dodecyl sulfate(SDS) |
1.0 |
| Sodium
myristate |
0.3 |
| Sodium
laurate |
0.1 |
| Sodium
palmitate |
<0.1 |
| Sodium
stearate |
<0.1 |
| Nonionic surfactants |
| Nonylphenol
ethoxylate(EO=10) |
<0.1 |
| Polyoxyethylene(EO=20)
sorbitan monolaurate |
<0.1 |
| Disodium
lauryl surlfosuccinate |
<0.1 |
| Benzenesulfonic
acid |
<0.1 |
| Phenol |
<0.1 |
| Toluene |
<0.1 |
| Xylene |
<0.1 |
The monoclonal antibody has a high specificity to LAS with various
alkyl chain length (n=8-12) and doesn't cross-react with other surfactants or
compounds of similar structure.
Kit Format
| Kit Format |
Comment |
| Microplate |
1 plate (96 wells)
& reagents |
Needs a microplate reader(450nm)
For multiple and simultaneous measurement |
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