 supersensitive BPA ELISA Kit |
 Bisphenol
A (BPA), principal raw material for polycarbonate and epoxy resins,
is suspected as one of the endocrine disrupting chemicals.
GC/MS, generally employed methods for quantitative BPA analysis,
requires expensive instrumentation as well as complex and time-consuming
extraction process with hazardous organic solvent.
 This test kit detects BPA in environment or in vitro
with high sensitivity with the aid of solid phase extraction.
Features
- The quantitative analysis ranges from 0.05-10μg/L(ppb), sensitive enough
to detect BPA in a specimen from river water, etc..
- A simple solid phase extraction is available
to measure samples with lower concentration.
- The total time for measurement is only 2.5 hours.
- BPA monoclonal antibody binds exclusively with BPA and does
not show cross-reaction with other chemicals with similar structures.
- The amount of harmful solvent can be minimized.
| Standard Curve of BPA |
|
|
| Cross-Reactivity Pattern |
| Compounds |
Cross Reactivity(%) |
| Bisphenol A (BPA) |
100 |
| Diethylhexylphthalate (DEHP) |
<0.05 |
| Nonylphenol (NP) |
0.19 |
| 17β-Estradiol (E2) |
<0.05 |
| Estrone (E1) |
<0.05 |
| Linear alkylbenzene sulfonate (LAS) |
<0.05 |
| Alkylphenol Ethoxylate (APE) |
<0.05 |
| Alkyl Ethoxylate (AE) |
<0.05 |
| Humic acid Na |
<0.05 |
|
| . |
Kit Format
| Kit Format |
Comment |
| Microplate |
1 plate (96 wells)& reagents |
Needs a microplate reader (450nm)
For multiple and simultaneous measurement |
Simple Solid Phase
Extraction (example)
- Adjust the pH of the sample to 3.5 with 1M HCl.
- Wash out C18 solid phase cartridge with MeOH and distilled
water; then pour the sample through the cartridge.
- Rinse the cartridge with water, then elute the mixture of
aceton and MeOH at the ratio of 1:1.
- Dry the sample with nitrogen gas, then adjust MeOH concentration
at 10% for measurement.
For research use only. Not for diagnostic use.
|
