supersensitive BPA ELISA Kit

Bisphenol A (BPA), principal raw material for polycarbonate and epoxy resins, is suspected as one of the endocrine disrupting chemicals.
GC/MS, generally employed methods for quantitative BPA analysis, requires expensive instrumentation as well as complex and time-consuming extraction process with hazardous organic solvent.

This test kit detects BPA in environment or in vitro with high sensitivity with the aid of solid phase extraction.


  • The quantitative analysis ranges from 0.05-10μg/L(ppb), sensitive enough to detect BPA in a specimen from river water, etc..
  • A simple solid phase extraction is available to measure samples with lower concentration.
  • The total time for measurement is only 2.5 hours.
  • BPA monoclonal antibody binds exclusively with BPA and does not show cross-reaction with other chemicals with similar structures.
  • The amount of harmful solvent can be minimized.

Standard Curve of BPA
Cross-Reactivity Pattern
Compounds Cross Reactivity(%)
Bisphenol A (BPA) 100
 Diethylhexylphthalate (DEHP) <0.05
 Nonylphenol (NP) 0.19
 17β-Estradiol (E2) <0.05
 Estrone (E1) <0.05
 Linear alkylbenzene sulfonate (LAS) <0.05
 Alkylphenol Ethoxylate (APE) <0.05
 Alkyl Ethoxylate (AE) <0.05
 Humic acid Na <0.05

Kit Format

Kit Format Comment
Microplate 1 plate (96 wells)& reagents Needs a microplate reader (450nm)
For multiple and simultaneous measurement

Simple Solid Phase Extraction (example)

  • Adjust the pH of the sample to 3.5 with 1M HCl.
  • Wash out C18 solid phase cartridge with MeOH and distilled water; then pour the sample through the cartridge.
  • Rinse the cartridge with water, then elute the mixture of aceton and MeOH at the ratio of 1:1.
  • Dry the sample with nitrogen gas, then adjust MeOH concentration at 10% for measurement.

For research use only. Not for diagnostic use.

The details of ECOLOGIENA are here.